Protein expression and purification Mammalian cells HeLa

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Get tips on using Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy to perform ROS assay cell type - H9c2 rat cardiomyocytes

Products Thermo Fisher Scientific Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy

Get tips on using INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI to perform Bacterial cell culture media Enterococcus faecium

Products BIOMED INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI

Get tips on using INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI to perform Bacterial cell culture media Enterococcus faecalis

Products BIOMED INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #13082 to perform Autophagy assay cell type - RAW 264.7

Products Cell Signaling Technology LC3A/B (D3U4C) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #13082

Get tips on using Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit to perform Cell cycle assay human - OVCAR-5

Products Thermo Fisher Scientific Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit

Get tips on using ViralSEQ™ Quantitative Sf-Rhabdovirus Kit with PrepSEQ™ Nucleic Acid Sample Prep Kit to perform Cell Culture Contamination Detection Kit Virus

Products Thermo Fisher Scientific ViralSEQ™ Quantitative Sf-Rhabdovirus Kit with PrepSEQ™ Nucleic Acid Sample Prep Kit

I intend to use iScript cDNA Synthesis Kit in order to synthesize cDNA for qPCR. I have confirmed that my RNA is pure however, according to my extraction protocol I have suspended the RNA in TE buffer containing 1mM EDTA. Will the presence of EDTA have an effect on cDNA synthesis?

Discussions Will presence of EDTA effect cDNA synthesis

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